Figure 3: Nitric oxide generation in the media of cell cultures subjected to oxygen-glucose deprivation. b.End. 3 cells were exposed to 4 h oxygen-glucose deprivation followed by 24 h reperfusion. Estimates of nitric oxide accumulation (nitrate) from culture media were made using the Griess reagent. Nonsignificant decreases in nitric oxide accumulation were detected in the media of oxygen-glucose deprivation exposed cultures (4 h) compared to uninjured controls, but this was not affected by the addition of 100 nM C5a (4 h + C5a). OGD: Oxygen-glucose deprivation, NO: Nitric oxide, Con: Controls